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KMID : 0380019950100010078
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 1 p.78 ~ p.87
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Citric Acid Production Using Encapsulated Aspergillus niger
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Abstract
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The encapsulated A. niger grew up inside the capsule and mycelia penetrated through the pore of the capsule membrane. The mycelia on the capsule wall became loose when the carbon source and oxygen were deficient in the medium. On the contrary, the production rate increased and mycelia made a lump tightly when the carbon source and oxygen were sufficient. Namely, number of proper capsule of unit volume in the medium was existed. The phenomenon which was swelled of capsule membrane in cultivation could prevented by adding CaCl2 into the medium. According to the time adding CaC12 into the medium,the production rate of citric acid was influenced. In case of adding CaC12 into the medium at 7th day cultivation, the production yield of citric acid was increased about 40 percent higher than that of adding CaCl2 initially. The production yield of citric acid using encapsulated A. niger of flask culture was influenced with oxygen supply. The production yield of citric acid (dp/ds) of the flask culture was increased 3.88 time by using T-flask instead of parafilm sealed flask. Therefore, the productivity and consumption rate concering production which was taken carbon source were increased when oxygen supply was sufficent. The production of citric acid using encapsulated A. niger was increased average 30 percent higher than that of bead in between 6th and 13th day cultivation.
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